Mouse Anti-SUDV GP mAb (16F6)

Catalog #:

0280-001

Size:    

100 ug

Applications:  

ELISA

Clonality:        

Monoclonal Antibody

Host:

Mouse

Target:

Sudan Virus

Description:

Purified mouse monoclonal antibody 16F6 that binds to a conformation epitope on Sudan virus (SUDV) glycoprotein (GP). This antibody demonstrates protection against SUDV both in vitro and in vivo (Dias et al., 2011 Nov 20, Nat Struct Mol Biol. 18 (12): 1424-1427).

Immunogen:

Venezuelan equine encephalitis virus replicons encoding SUDV GP and gamma-radiationinactivated SUDV-Boniface were used to generate the original mouse monoclonal antibody.

Supplied:

100 µg is supplied in phosphate buffered saline at a concentration of 1.315 mg/mL. No preservative is added.

Clonality:

Monoclonal of the IgG1 isotype

Relevance:

The antibody can be used as a positive control for plaque reduction neutralization test (PRNT) and neutralization assay using SUDV-GP pseudotyped VSV.

ELISA:

Assay-dependent dilution.

WB:

Not recommended because 16F6 recognizes conformational epitope.

Cross Reactivity:

In ELISA: No cross-reactivity was observed to Ebola virus (EBOV) or Marburg virus (MARV) virus-like particles (VLP).

Storage:

2-3 weeks +4°C, -20°C long term