Advancements in Size-Exclusion Chromatography: The Superose® 6 Increase 10/300 GL 1 ea Column

Chromatography columns are fundamental components in the separation and purification processes used in biochemical and molecular biology research. One exemplary tool in this category is the Superose® 6 Increase 10/300 GL 1 ea column, which is pivotal in size-exclusion chromatography (SEC) for the separation and analysis of biomolecules based on size. This high-performance column features a 10 mm inner diameter and a 300 mm height, packed with Superose® 6 Increase resin. This advanced agarose-based gel filtration medium offers high resolution and increased capacity, making it suitable for a broad range of biomolecules, from small peptides to large protein complexes.

Key Features and Applications

Resin Type:

Superose® 6 Increase Resin: This resin is engineered for superior separation efficiency, providing clear and distinct separation of biomolecular species. Its advanced design ensures that even complex mixtures are resolved with high precision.

Column Dimensions:

Inner Diameter: 10 mm

Height: 300 mm
Material: Glass (indicated by GL), which ensures chemical compatibility and visibility of the separation process.


Enhanced Resolution: The Superose® 6 Increase resin allows for clearer and more distinct separation of biomolecular species.
Increased Capacity: The column supports higher sample loads without compromising resolution, making it suitable for both analytical and preparative applications.

Gel chromatography of CaL on a Superose 6 10/300 using a FPLC-AKTA system.



1.Protein Purification:

The Superose® 6 Increase 10/300 GL column is ideal for isolating specific proteins from complex mixtures. Its high resolution ensures that proteins are purified with high precision, which is essential for subsequent biochemical analyses.

2.Molecular Weight Determination:

This column is frequently used to determine the molecular weights of proteins and other macromolecules. By separating molecules based on their size, researchers can accurately assess their molecular weight and gain insights into their structural and functional properties.

3.Complex Sample Analysis:

The column is highly effective in analyzing complex biological samples, such as cell lysates or biological fluids. It provides detailed size distribution profiles of biomolecules, aiding in understanding sample composition and behavior.

4.Biomolecular Interaction Studies:

The Superose® 6 Increase 10/300 GL column supports the study of interactions between biomolecules. It can separate complexes from free components, facilitating the investigation of binding properties and interaction dynamics, which is crucial for understanding molecular mechanisms in biological systems.


Operational Considerations

1.Flow Rate:

The recommended flow rate for the Superose® 6 Increase 10/300 GL column is typically around 0.5 to 1.0 mL/min, ensuring efficient separation without compromising resolution.

2.Sample Volume:

The column can handle sample volumes up to 500 µL, making it versatile for both small-scale analytical runs and larger preparative processes.


The Superose® 6 Increase 10/300 GL column is compatible with most chromatography systems and can be used with a variety of buffers, provided they do not exceed the chemical compatibility limits of the agarose-based resin.



The Superose® 6 Increase 10/300 GL 1 ea column represents a significant advancement in size-exclusion chromatography technology. Its high resolution, increased capacity, and versatility make it an essential tool for researchers in biochemistry and molecular biology. Whether for protein purification, molecular weight determination, or the analysis of complex samples, this column delivers reliable and high-quality results, supporting a wide range of scientific investigations aimed at unraveling the complexities of biological systems.


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Some Additional questions:


What is the difference between Superose 6 and Superose 6 increase? 
 The main difference between Superose® 6 and Superose® 6 Increase lies in their performance characteristics. Superose® 6 Increase columns are designed to offer improved resolution and capacity compared to the original Superose® 6 columns.

1.Resolution: Superose® 6 Increase columns typically provide higher resolution, allowing for clearer and more distinct separation of biomolecular species. This is particularly beneficial when analyzing complex mixtures or closely related compounds.

2.Capacity: Superose® 6 Increase columns have an increased capacity, meaning they can handle higher sample loads without compromising resolution. This makes them suitable for both analytical and preparative applications.

3.Particle Size: Superose® 6 Increase columns may have different particle sizes or pore sizes compared to Superose® 6 columns, contributing to their improved performance characteristics.

Overall, Superose® 6 Increase columns are considered an advancement over the original Superose® 6 columns, offering improved performance for a wide range of chromatographic applications.



How to clean superose 6 column?

Cleaning a Superose® 6 column, or any chromatography column, is essential for maintaining its performance and extending its lifespan. Here are general steps for cleaning a Superose® 6 column:

Flush with Buffer:

Flush the column with a compatible buffer (e.g., 20 mM Tris-HCl, pH 7.5) to remove any remaining sample.
Wash with Detergent:

Prepare a cleaning solution with a mild detergent (e.g., 0.1% Triton X-100) in the buffer.
Pass the cleaning solution through the column and allow it to sit in the column for a specified time (e.g., 30 minutes) to remove contaminants.
Rinse with Buffer:

Flush the column thoroughly with the buffer to remove the detergent and any remaining contaminants.

Store the column in a storage solution (e.g., 20% ethanol) to prevent microbial growth and maintain column integrity.
Always refer to the manufacturer's instructions for specific cleaning and storage recommendations for your Superose® 6 column.